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KMID : 0043320140370050645
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Archives of Pharmacal Research
2014 Volume.37 No. 5 p.645 ~ p.651
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Expression system for production of bioactive compounds, recombinant human adiponectin, in the silk glands of transgenic silkworms
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Shin Seul-Mee
Kim Bong-Yoon
Jeon Hyung-Yook
Lee Ae-Ri
Lee Sung-Won
Sung Seung-Hyun
Park Chan-Su
Lee Chong-Kil
Kong Hyun-Seok
Song Young-Cheon
Kim Kyung-Jae
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Abstract
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Adiponectin is an adipocyte hormone involved in glucose and lipid metabolism. The aim of this study was to develop a human adiponectin expression system in transgenic silkworm using a human adiponectin expression vector. The silk gland of the silkworm is a highly specialized organ that has the wonderful ability to synthesize and secrete silk protein. To express human adiponectin in the silk gland of transgenic silkworm, targeting vectors pB-A3-adiponectin-IRES-RFP and pB-Ser1-adiponectin-IRES-RFP were constructed and then introduced into the silkworm pupa. The transgenic silkworms were verified by PCR and then generated. The level of adiponectin in the transgenic silkworm was 6?10 ng/50 mg of freeze-dried powder, and western blotting using an antibody against human adiponectin demonstrated a specific band with a molecular weight of 30 kDa in the silkworm. These results showed that human adiponectin introduced into the silkworm genome was expressed successfully on a large-scale.
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KEYWORD
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Adiponectin, Bombyx mori, Transgenic silkworms, Silk glands
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